Our aim is to characterize selected plant P450s which are involved in the biosynthesis of secondary metabolites. For this approach the P450s will be expressed in recombinant microbial hosts to produce them in sufficient amounts and to investigate their substrate specificities and selectivities in vitro. Furthermore the activities towards selected substrates will be recapitulated in vivo with recombinant yeast.
- Expression and characterization of P450s and oxidosqualene cyclases involved in plant terpernoid biosynthesis
Our aim is to investigate selected cytochrome P450 enzymes and oxidosqualene cyclases of Arabidopsis thaliana, involved in terpenoid biosynthesis. As heterologous hosts Escherichia coli and Pichia pastoris will be used to produce recombinant enzymes at concentrations, sufficient for their characterization in vitro. Additionally, P. pastoris should be tested as potential producer of plant terpenoids.