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Comparative biochemical characterization of mammalian-derived CYP11A1s with cholesterol side-chain cleavage activities (Kopie 1)

Shooting von Ivo Mayr: Forschung und Labore

Many steroids have become important drugs because they regulate various cellular processes in human body. Selectively oxyfunctionalized steroid derivatives exhibit higher or different biological activity and are therefore attractive for the pharmaceutical industry.  In this study the scientists comparatively investigated ten CYP11A1 enzymes of different origins with the target to identify CYP11A1s with higher cholesterol side-chain cleavage activity. As a result, two new CYP11A1s with cholesterol side-chain cleavage function in vitro were characterised, expanding the toolbox of this valuable biocatalysts. The publication recently appeared in Journal of Steroid Biochemistry and Molecular Biology. Below you can read the abstract. The link to the whole publication can be found in the reference at the bottom.

"Steroid drugs, the second largest class of pharmaceuticals after antibiotics, have shown significant anti-inflammatory, anti-allergic, and endocrine-regulating effects. A group of cytochrome P450 enzymes, namely, CYP11A1 isoenzymes from different organisms are capable of converting cholesterol into pregnenolone, which is a pivotal reaction in both steroid metabolism and (bio)synthetic network of steroid products. However, the low activity of CYP11A1s greatly restricts the industrial application of these cholesterol side-chain cleavage enzymes. Herein, we investigate ten CYP11A1 enzymes of different origins and in vitro characterize two CYP11A1s with a relatively higher expression level from Capra hircus and Sus scrofa, together with the CYP11A1s from Homo sapiens and Bos taurus as references. Towards five selected sterol substrates with different side chain structures, S. scrofa CYP11A1 displays relatively higher activities. Through redox partners combination screening, we reveal the optimal redox partner pair of S. scrofa adrenodoxin and C. hircus adrenodoxin reductase. Moreover, the semi-rational mutagenesis for the active sites and substrate entrance channels of human and bovine CYP11A1s is performed based on comparative analysis of their crystal structures. The mutant mBtCYP11A1-Q377A derived from mature B. taurus CYP11A1 shows a 1.46 times higher activity than the wild type enzyme. These results not only demonstrate the tunability of the highly conserved CYP11A1 isoenzymes, but also lay a foundation for the following engineering efforts on these industrially relevant P450 enzymes."

Liu R, Pan Y, Wang N, Tang D, Urlacher VB, Li S, 2023, Comparative biochemical characterization of mammalian-derived CYP11A1s with cholesterol side-chain cleavage activities, Journal of Steroid Biochemistry and Molecular Biology, 229:106268, https://doi.org/10.1016/j.jsbmb.2023.106268

All publications of the biochemistry II can be found here!

Kategorie/n: BC Urlacher